Urine proteome study for the evaluation of age dynamics in healthy men

L. Kh Pastushkova, A. S. Kononikhin, E. S. Tiys, I. V. Dobrokhotov, V. A. Ivanisenko, E. N. Nikolaev, I. M. Larina, I. A. Popov

Результат исследований: Вклад в журналСтатьярецензирование

Аннотация

We investigated the age dynamics of proteomic profile of urine in 52 healthy men aged 18 to 51 years. A special sample preparation was performed, followed by liquid chromatography-mass spectrometry. Liquid chromatography-mass spectrometry of the minor proteins was performed on a nano-HPLC Agilent 1100 system («Agilent Technologies Inc.», USA) in combination with a LTQ-FT Ultra mass spectrometer («Thermo Electron», Germany). A total of 259 proteins were identified. According to the TiGER database, a tissue origin was established for 141 proteins and identified 715 processes in which they participate. We found a significant positive correlation with age, the number of proteins (R=0,566; p-value=1,24E-05) and the weight of proteins (R=0,45; p value=8,17E-04). Identified 23 proteins were significantly more frequent in the urine of subjects with increasing age (p<0,05), and only one protein - RGSL, Regulator of G protein signaling protein-like (MW 125.69) - less frequently.

Язык оригиналаАнглийский
Страницы (с-по)694-700
Число страниц7
ЖурналAdvances in gerontology = Uspekhi gerontologii
Том28
Номер выпуска4
СостояниеОпубликовано - 1 янв. 2015
Опубликовано для внешнего пользованияДа

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