The role of RNA polymerase σ subunit in promoter-independent initiation of transcription

Nikolay Zenkin, Konstantin Severinov

Результат исследований: Вклад в журналСтатьярецензирование

23 Цитирования (Scopus)


In bacteria, initiation of transcription depends on the RNA polymerase σ subunit, which brings catalytically proficient RNA polymerase core to promoters by binding to specific DNA elements located upstream of the transcription start point. Here, we study σ-dependent synthesis of a transcript that is used to prime replication of the single-stranded genome of bacteriophage M13. We show that, in this system, σ plays no role in DNA recognition, which is accomplished solely through RNA polymerase core interaction with DNA downstream of the transcription start point. However, σ is required for full-sized transcript synthesis by allowing RNA polymerase core to escape into productive elongation. RNA polymerase σ may play a similar role during replication primer synthesis in other bacterial mobile elements whose life cycle involves a single-stranded DNA stage.

Язык оригиналаАнглийский
Страницы (с-по)4396-4400
Число страниц5
ЖурналProceedings of the National Academy of Sciences of the United States of America
Номер выпуска13
СостояниеОпубликовано - 30 мар. 2004
Опубликовано для внешнего пользованияДа


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