Regulated communication between the upstream face of RNA polymerase and the β′ subunit jaw domain

Siva R. Wigneshweraraj, Patricia C. Burrows, Sergei Nechaev, Niicolay Zenkin, Konstantin Severinov, Martin Buck

Результат исследований: Вклад в журналСтатьярецензирование

28 Цитирования (Scopus)

Аннотация

We used bacteriophage T7-encoded transcription inhibitor gene protein 2 (gp2) as a probe to study the contribution of the Escherichia coli RNA polymerase (RNAP) β′ subunit jaw domain-the site of gp2 binding-to activator and ATP hydrolysis-dependent open complex formation by the σ54-RNAP. We show that, unlike σ70-dependent transcription, activated transcription by σS4-RNAP is resistant to gp2. In contrast, activator and ATP hydrolysis-independent transcription by σ54-RNAP is highly sensitive to gp2. We provide evidence that an activator- and ATP hydrolysis-dependent conformational change involving the β′ jaw domain and promoter DNA is the basis for gp2-resisiant transcription by σ54-RNAP. Our results establish that accessory factors bound to the upstream face of the RNAP, communicate with the β′ jaw domain, and that such communication is subjected to regulation.

Язык оригиналаАнглийский
Страницы (с-по)4264-4274
Число страниц11
ЖурналEMBO Journal
Том23
Номер выпуска21
DOI
СостояниеОпубликовано - 27 окт. 2004
Опубликовано для внешнего пользованияДа

Fingerprint

Подробные сведения о темах исследования «Regulated communication between the upstream face of RNA polymerase and the β′ subunit jaw domain». Вместе они формируют уникальный семантический отпечаток (fingerprint).

Цитировать