Novel homo Yin-Yang probes improve sensitivity in RT-qPCR detection of low copy HIV RNA

Valentina M. Farzan, Maksim V. Kvach, Ilya O. Aparin, Dmitry E. Kireev, Tatiana A. Prikazchikova, Alexey V. Ustinov, Vadim V. Shmanai, German A. Shipulin, Vladimir A. Korshun, Timofei S. Zatsepin

    Результат исследований: Вклад в журналСтатьярецензирование

    8 Цитирования (Scopus)


    Nucleic acids labeled with a fluorophore/quencher pair are widely used as probes in biomedical research and molecular diagnostics. Here we synthesized novel DNA molecular beacons double labeled with the identical dyes (R6G, ROX and Cy5) at 5′- and 3′-end and studied their photo physical properties. We demonstrated that fluorescence quenching by formation of the homo dimer exciton in such molecular beacons allows using them in homogeneous assays. Further, we developed and evaluated homo Yin-Yang DNA probes labeled with identical dyes and used them for detection of low copy HIV RNA by RT-qPCR. They demonstrated improved sensitivity (LLQ: 10 vs 30 copies mL-1) in comparison to commercially available Abbott RealTime HIV-1 kit based on VIC-BHQ dyes both for model mixtures (naive human plasma with added deactivated HIV-1 virus) and for preliminarily confirmed 36 clinical samples (4 vs 1 positive ones for low-copy samples).

    Язык оригиналаАнглийский
    Страницы (с-по)226-232
    Число страниц7
    СостояниеОпубликовано - 1 мар. 2019


    Подробные сведения о темах исследования «Novel homo Yin-Yang probes improve sensitivity in RT-qPCR detection of low copy HIV RNA». Вместе они формируют уникальный семантический отпечаток (fingerprint).