Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays

Sarah A. Michaud, Nicholas J. Sinclair, Helena Pětrošová, Andrea L. Palmer, Adam J. Pistawka, Suping Zhang, Darryl B. Hardie, Yassene Mohammed, Azad Eshghi, Vincent R. Richard, Albert Sickmann, Christoph H. Borchers

Результат исследований: Вклад в журналСтатьярецензирование

15 Цитирования (Scopus)

Аннотация

Mouse is the predominant experimental model for the study of human disease due, in part, to phylogenetic relationship, ease of breeding, and the availability of molecular tools for genetic manipulation. Advances in genome-editing methodologies, such as CRISPR-Cas9, enable the rapid production of new transgenic mouse strains, necessitating complementary high-throughput and systematic phenotyping technologies. In contrast to traditional protein phenotyping techniques, multiple reaction monitoring (MRM) mass spectrometry can be highly multiplexed without forgoing specificity or quantitative precision. Here we present MRM assays for the quantitation of 500 proteins and subsequently determine reference concentration values for plasma proteins across five laboratory mouse strains that are typically used in biomedical research, revealing inter-strain and intra-strain phenotypic differences. These 500 MRM assays will have a broad range of research applications including high-throughput phenotypic validation of novel transgenic mice, identification of candidate biomarkers, and general research applications requiring multiplexed and precise protein quantification.

Язык оригиналаАнглийский
Номер статьи78
ЖурналCommunications Biology
Том1
Номер выпуска1
DOI
СостояниеОпубликовано - 1 дек. 2018
Опубликовано для внешнего пользованияДа

Fingerprint

Подробные сведения о темах исследования «Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays». Вместе они формируют уникальный семантический отпечаток (fingerprint).

Цитировать