Escherichia coli ribosomes as a model for testing new photoactivated tRNA analogs containing 6-thioguanosine residues

I. N. Lavrik, P. V. Sergiev, A. A. Bogdanov, R. A. Zimmermann, O. A. Dontsova

Результат исследований: Вклад в журналСтатьярецензирование

1 Цитирования (Scopus)

Аннотация

A comparative study of photoreactive tRNA derivatives containing a 6-thioguanosine or diazirine derivative of 5-methyleneaminouridine was performed using Escherichia coli ribosomes. The conditions of producing functional tRNAs containing photoreactive residues have been selected using T7 RNA polymerase transcription. Photochemical modification was conducted upon binding the corresponding tRNA Phe transcripts carrying the photoreactive residues of nucleotide analogs to the P site of the ribosome. The results of modification were analyzed using oligonucleotide-directed hydrolysis of rRNA by RNase H, as well as reverse transcription. It is shown that 6-thioguanosine is a promising agent for studying RNA-protein complexes by photochemical crosslinking methods.

Язык оригиналаАнглийский
Страницы (с-по)799-805
Число страниц7
ЖурналMolecular Biology
Том38
Номер выпуска5
DOI
СостояниеОпубликовано - сент. 2004
Опубликовано для внешнего пользованияДа

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