Entrapment of α-chymotrypsin into hollow polyelectrolyte microcapsules

Olga P. Tiourina, Alexei A. Antipov, Gleb B. Sukhorukov, Natalia I. Larionova, Yuri Lvov, Helmuth Möhwald

Результат исследований: Вклад в журналСтатьярецензирование

Аннотация

Stable hollow polyelectrolyte capsules were produced by the layer-by-layer assembling of non-biodegradable polyelectrolytes - poly(allylamine) and poly(styrenesulfonate) on melamine formaldehyde microcores followed by the core decomposition at low pH. A proteolylic enzyme, α-chymotrypsin, was encapsulated into these microcapsules with high yields of up to 100%. The encapsulation procedure was based on the protein adsorption onto the capsule shells and on the pH-dependent opening and closing of capsule wall pores. The protein in the capsules retained a high activity, and thermo- and storage stability. The nanostructured polyelectrolyte shell protected the proteinase from a high molecular weight inhibitor. Such enzyme-loaded capsules can be used as microreactors for biocatalysis.

Язык оригиналаАнглийский
Страницы (с-по)209-214
Число страниц6
ЖурналMacromolecular Chemistry and Physics
Том202
Номер выпуска11
СостояниеОпубликовано - 4 авг. 2001
Опубликовано для внешнего пользованияДа

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