Cross-linking applications in structural proteomics

Evgeniy V. Petrotchenko, Jason J. Serpa, Christoph H. Borchers

Результат исследований: Глава в книге, отчете, сборнике статейГлаварецензирование

Аннотация

The overall design of a mass spectrometry (MS) cross-linking experiment needs to take into account the complexity of the protein system studied, the desired level of structural information that needs to be obtained, the mutual compatibility of each successive step, and the feasibility of the MS and data analysis of the processed sample. The initial step in a cross-linking experiment involves performing a cross-linking reaction on the protein(s) of interest, and several factors need to be taken into consideration. The goal of MS analysis of the cross-links is a confident identification of the cross- linked peptides and it therefore relies on accurate determination of which pairs of sites are cross-linked. Applications of cross-linking for the structural analyses of proteins can vary depending on the level of the protein system studied and structural questions asked. Single proteins, protein complexes, and whole protein-protein interaction networks can be the subject of cross-linking analyses.

Язык оригиналаАнглийский
Название основной публикацииProteomics for Biological Discovery
Издательwiley
Страницы175-196
Число страниц22
ISBN (электронное издание)9781119081661
ISBN (печатное издание)9781118279243
DOI
СостояниеОпубликовано - 1 янв. 2019
Опубликовано для внешнего пользованияДа

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