Covalent binding of modified nucleic acids to proteins as a method for investigation of specific protein-nucleic acid interactions

T. S. Zatsepin, N. G. Dolinnaya, E. A. Kubareva, M. G. Ivanovskaya, V. G. Metelev, T. S. Oretskaya

Результат исследований: Вклад в журналОбзорная статьярецензирование

5 Цитирования (Scopus)

Аннотация

Methods for specific covalent binding of modified nucleic acids to proteins and enzymes with conservation of their native structures are discussed. Possible applications of synthetic nucleic acids containing reactive (nucleophilic and electrophilic) groups and groups that can be photoactivated for testing protein-nucleic acid contacts and studying protein functioning are considered. The structures of protein-nucleic acid conjugates formed in the enzymatic reactions are described. Methods for the isolation and analysis of covalently bound protein-nucleic acid complexes are generalised. The main attention is given to methods for determination of protein sites attached to nucleic acids. The bibliography includes 226 references.

Язык оригиналаАнглийский
Страницы (с-по)77-95
Число страниц19
ЖурналRussian Chemical Reviews
Том74
Номер выпуска1
DOI
СостояниеОпубликовано - 2005
Опубликовано для внешнего пользованияДа

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