Application of spiroplasma melliferum proteogenomic profiling for the discovery of virulence factors and pathogenicity mechanisms in host-associated spiroplasmas

Dmitry Alexeev, Elena Kostrjukova, Alexander Aliper, Anna Popenko, Nikolay Bazaleev, Alexander Tyakht, Oksana Selezneva, Tatyana Akopian, Elena Prichodko, Ilya Kondratov, Mikhail Chukin, Irina Demina, Maria Galyamina, Dmitri Kamashev, Anna Vanyushkina, Valentina Ladygina, Sergei Levitskii, Vasily Lazarev, Vadim Govorun

Результат исследований: Вклад в журналСтатьярецензирование

36 Цитирования (Scopus)

Аннотация

To date, no genome of any of the species from the genus Spiroplasma has been completely sequenced. Long repetitive sequences similar to mobile units present a major obstacle for current genome sequencing technologies. Here, we report the assembly of the Spiroplasma melliferum KC3 genome into 4 contigs, followed by proteogenomic annotation and metabolic reconstruction based on the discovery of 521 expressed proteins and comprehensive metabolomic profiling. A systems approach allowed us to elucidate putative pathogenicity mechanisms and to discover major virulence factors, such as Chitinase utilization enzymes and toxins never before reported for insect pathogenic spiroplasmas.

Язык оригиналаАнглийский
Страницы (с-по)224-236
Число страниц13
ЖурналJournal of Proteome Research
Том11
Номер выпуска1
DOI
СостояниеОпубликовано - 1 янв. 2012
Опубликовано для внешнего пользованияДа

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