Analysis of DNA-protein interactions in complexes of transcription factor NF-κB with DNA

A. S. Romanenkov, A. A. Ustyugov, T. S. Zatsepin, A. A. Nikulova, I. V. Kolesnikov, V. G. Metelev, T. S. Oretskaya, E. A. Kubareva

Результат исследований: Вклад в журналСтатьярецензирование

7 Цитирования (Scopus)

Аннотация

We have applied bioinformatic analysis of X-ray 3D structures of complexes of transcription factor NF-κB with DNAs. We determined the number of possible Van der Waals contacts and hydrogen bonds between amino acid residues and nucleotides. Conservative contacts in the NF-κB dimer-DNA complex composed of p50 and/or p65 NF-κB subunit and DNA sequences like 5′-GGGAMWTTCC-3′ were revealed. Based on these results, we propose a novel scheme for interactions between NF-κB p50 homodimer and the κB region of the immunoglobulin light chain gene enhancer (Ig-κB). We applied a chemical cross-linking technique to study the proximity of some Lys and Cys residues of NF-κB p50 subunit with certain reactive nucleotides into its recognition site. In all cases, the experimentally determined protein-DNA contacts were in good agreement with the predicted ones.

Язык оригиналаАнглийский
Страницы (с-по)1212-1222
Число страниц11
ЖурналBiochemistry (Moscow)
Том70
Номер выпуска11
DOI
СостояниеОпубликовано - нояб. 2005
Опубликовано для внешнего пользованияДа

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