A new method for normalization of the peptide retention times in chromatographic/mass-spectrometric experiments

D. M. Avtonomov, I. A. Agron, A. S. Kononikhin, I. A. Popov, E. N. Nikolaev

Результат исследований: Вклад в журналСтатьярецензирование

2 Цитирования (Scopus)

Аннотация

Proteomic studies with the use of mass spectrometry required comparison of different experimenttal data (for example, control with a pathology or labeled and unlabeled samples). Identification of chromatographic peaks of the same substance in different chromatograms (time normalization of chromatograms) is complicated if peptides are identified in different experiments according only to their exactly evaluated masses and retention times on a chromatographic column. Retention times of the same peptides would vary from one experiment to another due to inevitable differences in experimental chromatographic conditions (replacement of chromatographic columns, slight changes in flow rates of a mobile phase or in a solvent concentration, or in other conditions). We proposed a reliable method for selection of peaks that corre sponded to the same peptides from chromatography/mass spectra for the subsequent alignment of retention times (both a linear and some other monotone function).

Язык оригиналаАнглийский
Страницы (с-по)146-150
Число страниц5
ЖурналRussian Journal of Bioorganic Chemistry
Том37
Номер выпуска2
DOI
СостояниеОпубликовано - мар. 2011
Опубликовано для внешнего пользованияДа

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