Yellow and Orange Fluorescent Proteins with Tryptophan-based Chromophores

Nina G. Bozhanova, Mikhail S. Baranov, Karen S. Sarkisyan, Roman Gritcenko, Konstantin S. Mineev, Svetlana V. Golodukhina, Nadezhda S. Baleeva, Konstantin A. Lukyanov, Alexander S. Mishin

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Rapid development of new microscopy techniques exposed the need for genetically encoded fluorescent tags with special properties. Recent works demonstrated the potential of fluorescent proteins with tryptophan-based chromophores. We applied rational design and random mutagenesis to the monomeric red fluorescent protein FusionRed and found two groups of mutants carrying a tryptophan-based chromophore: with yellow (535 nm) or orange (565 nm) emission. On the basis of the properties of proteins, a model synthetic chromophore, and a computational modeling, we concluded that the presence of a ketone-containing chromophore in different isomeric forms can explain the observed yellow and orange phenotypes.

Original languageEnglish
Pages (from-to)1867-1873
Number of pages7
JournalACS Chemical Biology
Issue number7
Publication statusPublished - 21 Jul 2017
Externally publishedYes


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