Using photoactivatable fluorescent protein Dendra2 to track protein movement

Research output: Contribution to journalReview articlepeer-review

85 Citations (Scopus)

Abstract

Photoactivatable fluorescent proteins are capable of dramatic changes in fluorescent properties in response to specific light irradiation. For example, they can be converted from cyan to green, or from green to red, or from nonfluorescent to a brightly fluorescent state. Several types of such proteins were developed recently, and some of them are already becoming popular tools to study protein mobility. Here we provide detailed recommendations on application of the monomeric green-to-red photoconvertible fluorescent protein Dendra2 for protein tracking in living cultured cells.

Original languageEnglish
Pages (from-to)553-565
Number of pages13
JournalBioTechniques
Volume42
Issue number5
DOIs
Publication statusPublished - May 2007
Externally publishedYes

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