Usage of rRNA-methyltransferase for site-specific fluorescent labeling

O. V. Sergeeva, D. E. Burakovsky, P. V. Sergiev, T. S. Zatsepin, M. Tomkuviene, S. Klimasauskas, O. A. Dontsova

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The possibility of using an S-adenosylmethionine analog, i. e., pent-2-en-4-ynyl S-adenosylhomocysteine (AduEnYn), as an rRNA methyltransferase cofactor has been investigated. The conditions for the cycloaddition reaction of the fluorescent label to the S-adenosylmethionine analog were chosen. The functional activity of E. coli ribosomes was tested under different conditions. It was found that the introduction of the alkynyl radical occurred successfully and did not affect the functional activity of the ribosome; however, the inactivation of the ribosome occurred during the following cycloaddition reaction.

Original languageEnglish
Pages (from-to)88-93
Number of pages6
JournalMoscow University Chemistry Bulletin
Volume67
Issue number2
DOIs
Publication statusPublished - Mar 2012
Externally publishedYes

Keywords

  • cycloaddition reaction
  • E. coli ribosome
  • fluorescent labeling
  • methyltransferase

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