Urine proteome study for the evaluation of age dynamics in healthy men

L. Kh Pastushkova, A. S. Kononikhin, E. S. Tiys, I. V. Dobrokhotov, V. A. Ivanisenko, E. N. Nikolaev, I. M. Larina, I. A. Popov

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated the age dynamics of proteomic profile of urine in 52 healthy men aged 18 to 51 years. A special sample preparation was performed, followed by liquid chromatography-mass spectrometry. Liquid chromatography-mass spectrometry of the minor proteins was performed on a nano-HPLC Agilent 1100 system («Agilent Technologies Inc.», USA) in combination with a LTQ-FT Ultra mass spectrometer («Thermo Electron», Germany). A total of 259 proteins were identified. According to the TiGER database, a tissue origin was established for 141 proteins and identified 715 processes in which they participate. We found a significant positive correlation with age, the number of proteins (R=0,566; p-value=1,24E-05) and the weight of proteins (R=0,45; p value=8,17E-04). Identified 23 proteins were significantly more frequent in the urine of subjects with increasing age (p<0,05), and only one protein - RGSL, Regulator of G protein signaling protein-like (MW 125.69) - less frequently.

Original languageEnglish
Pages (from-to)694-700
Number of pages7
JournalAdvances in gerontology = Uspekhi gerontologii
Volume28
Issue number4
Publication statusPublished - 1 Jan 2015
Externally publishedYes

Keywords

  • aging
  • healthy people
  • mass-spectrometry
  • proteins
  • urine proteomics

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