Unraveling the mechanisms of chromatin fibril packaging

Alexey A. Gavrilov, Yuri Y. Shevelyov, Sergey V. Ulianov, Ekaterina E. Khrameeva, Pavel Kos, Alexander Chertovich, Sergey V. Razin

Research output: Contribution to journalComment/debate

12 Citations (Scopus)

Abstract

ABSTRACT: Recent data indicate that eukaryotic chromosomes are organized into Topologically Associating Domains (TADs); however, the mechanisms underlying TAD formation remain obscure. Based on the results of Hi-C analysis performed on 4 Drosophila melanogaster cell lines, we have proposed that specific properties of nucleosomes in active and repressed chromatin play a key role in the formation of TADs. Our computer simulations showed that the ability of “inactive” nucleosomes to stick to each other and the lack of such ability in “active” nucleosomes is sufficient for spatial segregation of these types of chromatin, which is revealed in the Hi-C analysis as TAD/inter-TAD partitioning. However, some Drosophila and mammalian TADs contain both active and inactive chromatin, a fact that does not fit this model. Herein, we present additional arguments for the model by postulating that transcriptionally active chromatin is extruded on the surface of a TAD, and discuss the possible impact of this organization on the enhancer-promoter communication and on the segregation of TADs.

Original languageEnglish
Pages (from-to)319-324
Number of pages6
JournalNucleus
Volume7
Issue number3
DOIs
Publication statusPublished - 3 May 2016
Externally publishedYes

Keywords

  • chromatin spatial structure
  • nucleosome
  • polymer simulations
  • topologically associating domains
  • transcription

Fingerprint

Dive into the research topics of 'Unraveling the mechanisms of chromatin fibril packaging'. Together they form a unique fingerprint.

Cite this