Twelve million resolving power on 4.7 T fourier transform ion cyclotron resonance instrument with dynamically harmonized cell - Observation of fine structure in peptide mass spectra

Igor A. Popov, Konstantin Nagornov, Gleb N.vladimirov, Yury I. Kostyukevich, Eugene N. Nikolaev

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Resolving power of about 12,000 000 at m/z 675 has been achieved on low field homogeneity 4.7 T magnet using a dynamically harmonized Fourier transform ion cyclotron resonance (FT ICR) cell. Mass spectra of the fine structure of the isotopic distribution of a peptide were obtained and strong discrimination of small intensity peaks was observed in case of resonance excitation of the ions of the whole isotopic cluster to the same cyclotron radius. The absence of some peaks from the mass spectra of the fine structure was explained basing on results of computer simulations showing strong ion cloud interactions, which cause the coalescence of peaks with m/z close to that of the highest magnitude peak. The way to prevent peak discrimination is to excite ion clouds of different m/z to different cyclotron radii, which was demonstrated and investigated both experimentally and by computer simulations. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)790-799
Number of pages10
JournalJournal of the American Society for Mass Spectrometry
Volume25
Issue number5
DOIs
Publication statusPublished - May 2014
Externally publishedYes

Keywords

  • Dynamic harmonization
  • FT-ICR
  • FTMS
  • Magnetic field
  • Simulation
  • Ultra-high mass resolution

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