Transcription regulation of the EcoRV restriction-modification system

Ekaterina Semenova, Leonid Minakhin, Ekaterina Bogdanova, Maxim Nagornykh, Anatoliy Vasilov, Tomasz Heyduk, Alexander Solonin, Marina Zakharova, Konstantin Severinov

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


When a plasmid containing restriction-modification (R-M) genes enters a naïve host, unmodified host DNA can be destroyed by restriction endonuclease. Therefore, expression of R-M genes must be regulated to ensure that enough methyltransferase is produced and that host DNA is methylated before the endonuclease synthesis begins. In several R-M systems, specialized Control (C) proteins coordinate expression of the R and the M genes. C proteins bind to DNA sequences called C-boxes and activate expression of their cognate R genes and inhibit the M gene expression, however the mechanisms remain undefined. Here, we studied the regulation of gene expression in the C protein-dependent EcoRV system. We map the divergent EcoRV M and R gene promoters and we define the site of C protein-binding that is sufficient for activation of the EcoRV R transcription.

Original languageEnglish
Pages (from-to)6942-6951
Number of pages10
JournalNucleic Acids Research
Issue number21
Publication statusPublished - 2005
Externally publishedYes


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