Toehold-Mediated Selective Assembly of Compact Discrete DNA Nanostructures

Vladimir A. Brylev, Alexey V. Ustinov, Vladimir B. Tsvetkov, Nikolay A. Barinov, Ilya O. Aparin, Ksenia A. Sapozhnikova, Yana Y. Berlina, Egor A. Kokin, Dmitry V. Klinov, Timofei S. Zatsepin, Vladimir A. Korshun

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Production of small discrete DNA nanostructures containing covalent junctions requires reliable methods for the synthesis and assembly of branched oligodeoxynucleotide (ODN) conjugates. This study reports an approach for self-assembly of hard-to-obtain primitive discrete DNA nanostructures - "nanoethylenes", dimers formed by double-stranded oligonucleotides using V-shaped furcate blocks. We scaled up the synthesis of V-shaped oligonucleotide conjugates using pentaerythritol-based diazide and alkyne-modified oligonucleotides using copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and optimized the conditions for "nanoethylene"formation. Next, we designed nanoethylene-based "nanomonomers"containing pendant adapters. They demonstrated smooth and high-yield spontaneous conversion into the smallest cyclic product, DNA tetragon aka "nano-methylcyclobutane". Formation of DNA nanostructures was confirmed using native polyacrylamide gel electrophoresis (PAGE) and atomic force microscopy (AFM) and additionally studied by molecular modeling. The proposed facile approach to discrete DNA nanostructures using precise adapter-directed association expands the toolkit for the realm of DNA origami.

Original languageEnglish
Pages (from-to)15119-15127
Number of pages9
JournalLangmuir
Volume36
Issue number49
DOIs
Publication statusPublished - 15 Dec 2020

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