Thermus thermophilus Bacteriophage ϕYS40 Genome and Proteomic Characterization of Virions

Tatyana Naryshkina, Jing Liu, Laurence Florens, Selene K. Swanson, Andrey R. Pavlov, Nadejda V. Pavlova, Ross Inman, Leonid Minakhin, Sergei A. Kozyavkin, Michael Washburn, Arcady Mushegian, Konstantin Severinov

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

We determined the sequence of the 152,372 bp genome of φ{symbol}YS40, a lytic tailed bacteriophage of Thermus thermophilus. The genome contains 170 putative open reading frames and three tRNA genes. Functions for 25% of φ{symbol}YS40 gene products were predicted on the basis of similarity to proteins of known function from diverse phages and bacteria. φ{symbol}YS40 encodes a cluster of proteins involved in nucleotide salvage, such as flavin-dependent thymidylate synthase, thymidylate kinase, ribonucleotide reductase, and deoxycytidylate deaminase, and in DNA replication, such as DNA primase, helicase, type A DNA polymerase, and predicted terminal protein involved in initiation of DNA synthesis. The structural genes of φ{symbol}YS40, most of which have no similarity to sequences in public databases, were identified by mass spectrometric analysis of purified virions. Various φ{symbol}YS40 proteins have different phylogenetic neighbors, including myovirus, podovirus, and siphovirus gene products, bacterial genes and, in one case, a dUTPase from a eukaryotic virus. φ{symbol}YS40 has apparently arisen through multiple acts of recombination between different phage genomes as well as through acquisition of bacterial genes.

Original languageEnglish
Pages (from-to)667-677
Number of pages11
JournalJournal of Molecular Biology
Volume364
Issue number4
DOIs
Publication statusPublished - 8 Dec 2006
Externally publishedYes

Keywords

  • bacteriophage
  • genome
  • proteomics
  • Thermus thermophilus
  • virion

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