Thermosipho activus sp. nov., a thermophilic, anaerobic, hydrolytic bacterium isolated from a deep-sea sample

Olga A. Podosokorskaya, Elizaveta A. Bonch-Osmolovskaya, Anne Godfroy, Sergey N. Gavrilov, Daria A. Beskorovaynaya, Tatyana G. Sokolova, Tatyana V. Kolganova, Stepan V. Toshchakov, Ilya V. Kublanov

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8 Citations (Scopus)

Abstract

A novel obligately anaerobic, extremely thermophilic, organotrophic bacterium, strain Rift-s3T, was isolated from a deep-sea sample containing Riftia pachyptila sheath from Guaymas Basin, Gulf of California. Cells of the novel isolate were rods, 0.3-0.8 µmm in width and 1.5-10 µmm in length, surrounded by a sheath-like structure (toga). Strain Rift-s3T grew at temperatures ranging from 44 to 75 °C, at pH 5.5 to 8.0, and with NaCl concentrations of 3 to 60 g l-1. Under optimum conditions (65 °C, pH 6.0, NaCl 25 g l-1), the doubling time was 30 min. The isolate was able to ferment mono-, oligo-and polysaccharides including cellulose, chitin, xylan and pectin, and proteins including β-keratins, casein and gelatin. Acetate, hydrogen and carbon dioxide were the main products of glucose fermentation. The G+C content of the DNA was 30 mol%. Phylogenetic analysis of 16S rRNA gene sequences showed the affiliation of strain Rift-s3T with the genus Thermosipho, with Thermosipho atlanticus Ob7T as the closest relative (96.5% 16S rRNA gene sequence similarity). Based on the phylogenetic analysis and physiological properties of the novel isolate we propose a novel species of the genus Thermosipho, Thermosipho activus sp. nov., with Rift-s3T (=DSM 26467T=VKM B-2803T) as the type strain.

Original languageEnglish
Pages (from-to)3307-3313
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume64
DOIs
Publication statusPublished - 1 Sep 2014
Externally publishedYes

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