The high-resolution mass spectrometry study of the protein composition of amyloid-like urine aggregates associated with preeclampsia

Victoria A. Sergeeva, Natalia V. Zakharova, Anna E. Bugrova, Natalia L. Starodubtseva, Maria I. Indeykina, Alexey S. Kononikhin, Vladimir E. Frankevich, Eugene N. Nikolaev

    Research output: Contribution to journalLetterpeer-review

    7 Citations (Scopus)

    Abstract

    The study of protein misfolding and post-translational processing abnormalities is a promising diagnostic approach for socially significant pathologies associated with the accumulation of abnormal forms of proteins. Recently, it was shown that amyloid-like aggregates can be observed in the urine of pregnant women with preeclampsia, which is the most severe hypertensive complication that can lead to fateful outcomes. The protein composition of urine aggregates may clarify the molecular mechanisms underlying the pathology and has not yet been studied in detail. Using a proteomic approach based on high-resolution mass spectrometry, we studied the protein composition of amyloid-like structures that aggregate in the presence of Congo red azo-dye in the urine of pregnant women with preeclampsia. Fragments of β-sheets of α-1-antitrypsin, complement 3, haptoglobin, ceruloplasmin, and trypstatin were identified as most likely targets for Congo red binding.

    Original languageEnglish
    Pages (from-to)158-161
    Number of pages4
    JournalEuropean Journal of Mass Spectrometry
    Volume26
    Issue number2
    DOIs
    Publication statusPublished - 1 Apr 2020

    Keywords

    • amyloid-like aggregates
    • Congo red
    • high-resolution mass spectrometry
    • preeclamsia
    • protein misfolding
    • Proteomics

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