The Escherichia coli Yej transporter is required for the uptake of translation inhibitor microcin C

Maria Novikova, Anastasia Metlitskaya, Kirill Datsenko, Teymur Kazakov, Alexey Kazakov, Barry Wanner, Konstantin Severinov

Research output: Contribution to journalArticlepeer-review

76 Citations (Scopus)

Abstract

Microcin C (McC), a peptide-nucleotide antibiotic, targets aspartyl-tRNA synthetase. By analyzing a random transposon library, we identified Escherichia coli mutants resistant to McC. Transposon insertions were localized to a single locus, yejABEF, which encodes components of a putative inner membrane ABC transporter. Analysis of site-specific mutants established that all four components of the transporter are required for McC sensitivity. Since aspartyl-tRNA synthetase in yej mutant extracts was fully sensitive to McC, we conclude that yej mutations interfere with McC uptake and that YejABEF is the only inner membrane transporter responsible for McC uptake in E. coli. Other substrates of YejABEF remain to be identified.

Original languageEnglish
Pages (from-to)8361-8365
Number of pages5
JournalJournal of Bacteriology
Volume189
Issue number22
DOIs
Publication statusPublished - Nov 2007
Externally publishedYes

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