The development of a method for direct mass spectrometric analysis of biological samples using porous samplers

R. E. Levin, M. A. Shamraeva, I. M. Larina, D. S. Bormotov, S. I. Pekov, A. S. Shivalin, S. V. Silkin, V. A. Eliferov, K. V. Bocharov, E. N. Nikolaev, I. A. Popov

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The paper presents a method for rapid multi-omics investigation of biological samples using polypropylene bulk porous samplers. The use of porous samplers makes it easy to collect samples from the surface of the skin, mucous membranes, and biological fluids even in a field, and the surfaces of wounds and injuries. Collected samples do not require special storage conditions, and the samplers are lightweight and very compact. They can be used to monitor the condition of cosmonauts before, during, and after the spaceflight with the same sample collection method. The analysis of biomaterial applied to the sampler is performed using direct mass spectrometry methods, similar to the dried blood spot technique that is already used in clinical practice. However, bulk porous samplers allow expanding the range of analytes ionization conditions, which increases the stability and reliability of the ionization process, which expands the variety of analyzed molecules. The proposed method can be used to study compounds of various classes, including proteins, lipids, and metabolites, to systematically monitor the status of people in extreme conditions (athletes, astronauts), or to study the condition of patients in the clinic.

Original languageEnglish
Pages (from-to)99-103
Number of pages5
JournalAviakosmicheskaya i Ekologicheskaya Meditsina
Volume55
Issue number1
DOIs
Publication statusPublished - 2021
Externally publishedYes

Keywords

  • Biomaterial sampling
  • Biomaterial storage
  • Dried blood spot
  • Health monitoring
  • Mass spectrometry

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