The activity of [60]fullerene derivatives bearing amine and carboxylic solubilizing groups against Escherichia coli: A comparative study

Dmitry G. Deryabin, Olga K. Davydova, Zulfiya Zh Yankina, Alexey S. Vasilchenko, Sergei A. Miroshnikov, Alexey B. Kornev, Anastasiya V. Ivanchikhina, Pavel A. Troshin

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26 Citations (Scopus)

Abstract

We report a comparative investigation of the antibacterial activity of two water-soluble fullerene derivatives bearing protonated amine (AF) and deprotonated carboxylic (CF) groups appended to the fullerene cage via organic linkers. The negatively charged fullerene derivative CF showed no tendency to bind to the bacterial cells and, consequently, no significant antibacterial activity. In contrast, the compound AF loaded with cationic groups showed strong and partially irreversible binding to the negatively charged Escherichia coli K12 TG1 cells and to human erythrocytes, also possessing negative zeta potential. Adsorption of AF on the bacterial surface was visualized by atomic force microscopy revealing the formation of specific clusters (AF aggregates) surrounding the bacterial cell. Incubation of E. coli K12 TG1 with AF led to a dose-dependent bactericidal effect with L D 50 = 79.1 μM. The presence of human erythrocytes in the test medium decreased the AF antibacterial activity. Thus we reveal that the water-soluble cationic fullerene derivative AF possesses promising antibacterial activity, which might be utilized in the development of novel types of chemical disinfectants.

Original languageEnglish
Article number907435
JournalJournal of Nanomaterials
Volume2014
DOIs
Publication statusPublished - 2014
Externally publishedYes

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