The σ subunit conserved region 3 is part of '5'-face' of active center of Escherichia coli RNA polymerase

K. Severinov, D. Fenyo, E. Severinova, A. Mustaev, B. T. Chait, A. Goldfarb, S. A. Darst

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55 Citations (Scopus)

Abstract

Ribonucleotide analogs bound in the initiating site of Escherichia coli RNA polymerase holoenzyme in open promoter complexes were cross-linked to the β and σ70 subunits. Using limited proteolysis and chemical degradation, the cross-link site in σ70 was mapped to a segment between amino acids Glu508 and Met561 containing the C-terminal part of conserved region 3. This result, when reconciled with genetic data on the interaction of σ70 conserved regions 2 and 4 with the -10 and -35 promoter regions, respectively, allows us to model the orientation of the σ70 subunit domains within the open promoter complex.

Original languageEnglish
Pages (from-to)20826-20828
Number of pages3
JournalJournal of Biological Chemistry
Volume269
Issue number33
Publication statusPublished - 1994
Externally publishedYes

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