The β subunit Rif-cluster I is only angstroms away from the active center of Escherichia coli RNA polymerase

K. Severinov, A. Mustaev, E. Severinova, M. Kozlov, S. A. Darst, A. Goldfarb

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

Ribonucleotide analogs bound in the initiating site of Escherichia coli RNA polymerase-promoter complex were cross-linked to the β subunit. Using limited proteolysis and chemical degradation, the cross.link was mapped to a segment of β between amino acids Val516 and Arg540. This region (Rif- cluster I) is known to harbor many rifampicin-resistant (Rif(R)) mutations. The results demonstrate that Rif-cluster I is part of the '5'-face' of the active center and provide structural basis for the long-known effects of Rif(R) mutations on transcription initiation, elongation, and termination.

Original languageEnglish
Pages (from-to)29428-29432
Number of pages5
JournalJournal of Biological Chemistry
Volume270
Issue number49
DOIs
Publication statusPublished - 1995
Externally publishedYes

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