Tetracenomycin X inhibits translation by binding within the ribosomal exit tunnel

Ilya A. Osterman, Maximiliane Wieland, Tinashe P. Maviza, Kseniya A. Lashkevich, Dmitrii A. Lukianov, Ekaterina S. Komarova, Yuliya V. Zakalyukina, Robert Buschauer, Dmitrii I. Shiriaev, Semen A. Leyn, Jaime E. Zlamal, Mikhail V. Biryukov, Dmitry A. Skvortsov, Vadim N. Tashlitsky, Vladimir I. Polshakov, Jingdong Cheng, Yury S. Polikanov, Alexey A. Bogdanov, Andrei L. Osterman, Sergey E. DmitrievRoland Beckmann, Olga A. Dontsova, Daniel N. Wilson, Petr V. Sergiev

    Research output: Contribution to journalArticlepeer-review

    17 Citations (Scopus)

    Abstract

    The increase in multi-drug resistant pathogenic bacteria is making our current arsenal of clinically used antibiotics obsolete, highlighting the urgent need for new lead compounds with distinct target binding sites to avoid cross-resistance. Here we report that the aromatic polyketide antibiotic tetracenomycin (TcmX) is a potent inhibitor of protein synthesis, and does not induce DNA damage as previously thought. Despite the structural similarity to the well-known translation inhibitor tetracycline, we show that TcmX does not interact with the small ribosomal subunit, but rather binds to the large subunit, within the polypeptide exit tunnel. This previously unappreciated binding site is located adjacent to the macrolide-binding site, where TcmX stacks on the noncanonical basepair formed by U1782 and U2586 of the 23S ribosomal RNA. Although the binding site is distinct from the macrolide antibiotics, our results indicate that like macrolides, TcmX allows translation of short oligopeptides before further translation is blocked. [Figure not available: see fulltext.].

    Original languageEnglish
    Pages (from-to)1071-1077
    Number of pages7
    JournalNature Chemical Biology
    Volume16
    Issue number10
    DOIs
    Publication statusPublished - Oct 2020

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