Template-Assisted Assembly of DNA Nanostructures from Branched Oligonucleotides

A. A. Fokina, Yu E. Poletaeva, E. A. Burakova, A. Yu Bakulina, T. S. Zatsepin, E. I. Ryabchikova, D. A. Stetsenko

Research output: Contribution to journalArticlepeer-review


Abstract: We have proposed previously a template-assisted method for 3D assembly of nucleic acid nanostructures. The method involved a branched oligonucleotide as a template and a building block, which incorporates non-nucleotidic linkers, in particular, branching foci connecting two or three oligonucleotide chains. In this work, we have attempted the synthesis of branched oligonucleotide templates for the assembly of a DNA tetrahedron and a DNA cube, a branched oligonucleotide building block (“starlet”), and studied the assembly of a DNA tetrahedron.

Original languageEnglish
Pages (from-to)700-712
Number of pages13
JournalRussian Journal of Bioorganic Chemistry
Issue number3
Publication statusPublished - May 2021


  • click chemistry
  • DNA nanotechnology
  • nucleic acid
  • photolabile protecting group
  • solid-phase synthesis
  • strain-promoted azide-alkyne cycloaddition (SPAAC)


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