Telomerase from Saccharomyces cerevisiae contains several protein subunits and may have different activities depending on the protein content

Andrey V. Petrov, Svetlana S. Dokudovskaya, Kirill A. Sokolov, Olga I. Lavrik, Alain Favre, Olga A. Dontsova, Alexey A. Bogdanov

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Telomerase is a ribonucleoprotein responsible for maintaining telomeres during the cell cycle . Here we describe a two-step purification procedure for the Saccharomyces cerevisiae telomerase complex. We have found that the properties (processivity, nuclease activity) of telomerase depend on the isolation procedure. Using a cross-linking approach, we have revealed several proteins that could be components of the telomerase complex. Furthermore, spectra of cross-linked proteins differ in processive and non-processive telomerase complexes. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)35-40
Number of pages6
JournalFEBS Letters
Volume436
Issue number1
DOIs
Publication statusPublished - 25 Sep 1998
Externally publishedYes

Keywords

  • Nuclease activity
  • Processivity
  • Telomerase
  • Yeast

Fingerprint

Dive into the research topics of 'Telomerase from Saccharomyces cerevisiae contains several protein subunits and may have different activities depending on the protein content'. Together they form a unique fingerprint.

Cite this