Techniques for screening translation inhibitors

Ilya A. Osterman, Alexey A. Bogdanov, Olga A. Dontsova, Petr V. Sergiev

Research output: Contribution to journalReview articlepeer-review

3 Citations (Scopus)

Abstract

The machinery of translation is one of the most common targets of antibiotics. The development and screening of new antibiotics usually proceeds by testing antimicrobial activity followed by laborious studies of the mechanism of action. High-throughput methods for new antibiotic screening based on antimicrobial activity have become routine; however, identification of molecular targets is usually a challenge. Therefore, it is highly beneficial to combine primary screening with the identification of the mechanism of action. In this review, we describe a collection of methods for screening translation inhibitors, with a special emphasis on methods which can be performed in a high-throughput manner.

Original languageEnglish
Article number22
JournalAntibiotics
Volume5
Issue number3
DOIs
Publication statusPublished - Sep 2016
Externally publishedYes

Keywords

  • Antibiotic
  • High throughput screening
  • Ribosome
  • Translation

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