Targeted quantitation of CVD-linked plasma proteins for biomarker verification and validation

Andrew J. Percy, Simon Byrns, Andrew G. Chambers, Christoph H. Borchers

Research output: Contribution to journalReview articlepeer-review

7 Citations (Scopus)

Abstract

Despite significant advances in treatment, cardiovascular disease (CVD) remains one of the leading causes of morbidity and mortality in developed and developing countries. Judicious monitoring of common risk factors has been unable to control this global epidemic, necessitating novel biomarkers for improved screening and earlier disease detection and management. Although numerous plasma proteins have been associated with CVD, only a few of these potential biomarkers have been validated for clinical use. Here we review the quantitative proteomic methods used to verify and validate new biomarker candidates in human plasma. These methods center on a bottom-up approach involving multiple or selected reaction monitoring, for targeted detection, with stable isotope-labeled standards, for peptide normalization. Also included are a discussion of future strategies for improved CVD protein biomarker verification and validation, recommendations for method translation to the clinic, and future projections for protein biomarker research.

Original languageEnglish
Pages (from-to)567-578
Number of pages12
JournalExpert Review of Proteomics
Volume10
Issue number6
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Cardiovascular disease
  • Multiple reaction monitoring
  • Plasma
  • Protein
  • Targeted quantitation

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