Targeted and Untargeted Proteomics Approaches in Biomarker Development

Constance A. Sobsey, Sahar Ibrahim, Vincent R. Richard, Vanessa Gaspar, Georgia Mitsa, Vincent Lacasse, René P. Zahedi, Gerald Batist, Christoph H. Borchers

    Research output: Contribution to journalReview articlepeer-review

    36 Citations (Scopus)

    Abstract

    An enormous amount of research effort has been devoted to biomarker discovery and validation. With the completion of the human genome, proteomics is now playing an increasing role in this search for new and better biomarkers. Here, what leads to successful biomarker development is reviewed and how these features may be applied in the context of proteomic biomarker research is considered. The “fit-for-purpose” approach to biomarker development suggests that untargeted proteomic approaches may be better suited for early stages of biomarker discovery, while targeted approaches are preferred for validation and implementation. A systematic screening of published biomarker articles using MS-based proteomics reveals that while both targeted and untargeted technologies are used in proteomic biomarker development, most researchers do not combine these approaches. i) The reasons for this discrepancy, (ii) how proteomic technologies can overcome technical challenges that seem to limit their translation into the clinic, and (iii) how MS can improve, complement, or replace existing clinically important assays in the future are discussed.

    Original languageEnglish
    Article number1900029
    JournalProteomics
    Volume20
    Issue number9
    DOIs
    Publication statusPublished - 1 May 2020

    Keywords

    • biomarker discovery
    • biomarker validation
    • clinical proteomics
    • mass spectrometry

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