[Systems biology approach to the functional role of enzymatic modification of bacterial ribosome].

P. V. Sergiev, I. A. Osterman, I. V. Prokhorova, M. V. Nesterchuk, O. V. Sergeeva, A. I. Golovina, I. A. Demina, M. A. Galiamina, M. V. Serebriakova, O. A. Dontsova

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

In this work we describe methodology for studying the role of bacterial ribosome modification in the regulation of gene expression. Ribosomal components modification influences translation efficiencies of certain mRNAs. Proteome analysis allows us to identify cellular protein composition change caused by ribosome modification gene knockout. Particular stage of gene expression responsible for certain protein concentration change could be found using reporter constructs. After identification of mRNA species, whose translation is influenced by ribosome modification we can determine exact mRNA region responsible for the observed changes. The developed methodology can be applied for studying other translational control mechanisms.

Original languageEnglish
Pages (from-to)81-90
Number of pages10
JournalBioorganicheskaia khimiia
Volume37
Issue number1
Publication statusPublished - Jan 2011
Externally publishedYes

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