Synthesis of β-amyloid fragment 5RHDSGY10 and its isomers with the use of new solid-phase peptide synthesis schedule

E. Yu Aleshina, N. V. Pyndyk, A. A. Mosia, M. A. Sanzhakov, O. N. Kharybin, E. N. Nikolaev, E. F. Kolesanova

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1 Citation (Scopus)

Abstract

Peptide RHDSGY that represents the fragment of human β-amyloid Zn-binding site and its isomers RH(D-Asp)SGY and RH(β-Asp)SGY have been prepared by solid-phase synthesis and analysed by HPLC and various mass-spectrometric methods. The problem of low yield of peptide RHDSGY and its isomers attributed to 9-fluorenylmethoxycarbonyl (Fmoc)-amino acids and/or formation of side-products as RH(Asp-imide)SGY and RHDSGY (instead of RH(β-Asp)SGY) was solved via selection of reagents for the removal of Fmoc groups from the growing peptide chain.

Original languageEnglish
Pages (from-to)184-191
Number of pages8
JournalBiomeditsinskaya Khimiya
Volume54
Issue number2
Publication statusPublished - 2008
Externally publishedYes

Keywords

  • 4-methyl-piperidine
  • Fmoc-group removal
  • Solid-phase peptide synthesis
  • β-amyloid
  • β-Asp

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