Synthesis and characterization of bacteriochlorin loaded magnetic nanoparticles (MNP) for personalized MRI guided photosensitizers delivery to tumor

P. V. Ostroverkhov, A. S. Semkina, V. A. Naumenko, E. A. Plotnikova, P. A. Melnikov, T. O. Abakumova, R. I. Yakubovskaya, A. F. Mironov, S. S. Vodopyanov, A. M. Abakumov, A. G. Majouga, M. A. Grin, V. P. Chekhonin, M. A. Abakumov

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Hypothesis: Hydrophobic bacteriochlorin based photosensitizer (PS) can be effectively immobilized on MNP covered by human serum albumin (HSA). PS loading into MNP protein shell allows solubilizing PS in water solution without altering its photodynamic activity. MNP@PS can serve as diagnostic tool for tracking PS delivery to tumor tissues by MRI. Experiments: Immobilization on MNP-HSA-PEG was performed by adding PS solution in organic solvents with further purification. MNP@PS were characterized by DLS, HAADF STEM and AFM. Absorbance and fluorescence measurements were used to assess PS photophysical properties before and after immobilization. MNP@PS internalization into CT26 cells was investigated by confocal microscopy in vitro and MRI/IVIS were used for tracking MNP@PS delivery to tumors in vivo. Findings: MNP@PS complexes were stable in water solution and retained PS photophysical activity. The length of side chain affected MNP@PS size, loading capacity and cell internalization. In vitro testing demonstrated MNP@PS delivery to cancer cells followed by photoinduced toxicity. In vivo studies confirmed that as-synthetized complexes can be used for MRI tracking over drug accumulation in tumors.

Original languageEnglish
Pages (from-to)132-141
Number of pages10
JournalJournal of Colloid and Interface Science
Volume537
DOIs
Publication statusPublished - 1 Mar 2019

Keywords

  • Bacteriochlorin
  • Cancer
  • Drug delivery
  • Magnetic nanoparticles
  • Magnetic resonance imagining
  • Personalized medicine
  • Photodynamic therapy

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