Surface-specific washing-free immunosensor for time-resolved cortisol monitoring

Sofia M. Safarian, Pavel A. Kusov, Sergey S. Kosolobov, Oksana V. Borzenkova, Artem V. Khakimov, Yuri V. Kotelevtsev, Vladimir P. Drachev

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Cortisol is a steroid hormone that regulates a wide range of vital processes. Its level changes with diurnal rhythm and reacts to stress. Measurement of cortisol levels is still a complex multi-step process. A reversible washing-free registration method is required. Here we describe metal-enhanced fluorescence assay based on a displacement of a dye labeled BSA-cortisol conjugate from the immune complex immobilized on the golden islands by free cortisol. This competitive approach allows time-resolved monitoring of the fluorescent signal, surface-enhanced by the gold film, and provides the possibility of continuous real-time cortisol monitoring based on the implantable surface-enhanced immunosensor, which was not demonstrated so far even in vitro.

Original languageEnglish
Article number122070
JournalTalanta
Volume225
DOIs
Publication statusPublished - 1 Apr 2021

Keywords

  • BSA-cortisol conjugate
  • Competitive immunoassay
  • Cortisol
  • Gold nanostructures
  • Metal enhanced fluorescence
  • Real-time measurement

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