Study of Chloroplast DNA Polymorphism in the Sunflower (Helianthus L.)

N. V. Markina, A. V. Usatov, M. D. Logacheva, K. V. Azarin, C. F. Gorbachenko, I. V. Kornienko, V. A. Gavrilova, V. E. Tihobaeva

Research output: Contribution to journalArticlepeer-review

Abstract

The polymorphism of microsatellite loci of chloroplast genome in six Helianthus species and 46 lines of cultivated sunflower H. annuus (17 CMS lines and 29 Rf-lines) were studied. The differences between species are confined to four SSR loci. Within cultivated forms of the sunflower H. annuus, the polymorphism is absent. A comparative analysis was performed on sequences of the cpDNA inbred line 3629, line 398941 of the wild sunflower, and the American line HA383 H. annuus. As a result, 52 polymorphic loci represented by 27 SSR and 25 SNP were found; they can be used for genotyping of H. annuus samples, including cultural varieties: twelve polymorphic positions, of which eight are SSR and four are SNP.

Original languageEnglish
Pages (from-to)873-880
Number of pages8
JournalGenetika
Volume51
Issue number8
DOIs
Publication statusPublished - 1 Aug 2015
Externally publishedYes

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