Structure-based cross-linking of NF-κB p50 homodimer and decoy bearing a novel 2′-disulfide trapping site

Valeri Metelev, Andrey Romanenkov, Elena Kubareva, Evgeniy Zubin, Nikolai Polouchine, Timofei Zatsepin, Nikolai Molochkov, Tatiana Oretskaya

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Double-stranded oligodeoxyribonucleotides with engineered disulfide units were successfully used for covalent trapping of cysteine containing proteins. In particular, an efficient cross-linking of NF-κB p50 homodimer to a sequence-specific decoy was demonstrated. The results suggest that the synthetic oligonucleotides bearing a novel 2′-disulfide trapping site can be used as new tools to study and manipulate biological systems.

Original languageEnglish
Pages (from-to)654-658
Number of pages5
JournalIUBMB Life
Volume58
Issue number11
DOIs
Publication statusPublished - 1 Nov 2006
Externally publishedYes

Keywords

  • Cross-linking
  • Modified oligonucleotides
  • NF-κB p50 homodimer
  • Thiol-disulfide exchange

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