Structure-activity analysis of microcin J25: Distinct parts of the threaded lasso molecule are responsible for interaction with bacterial RNA polymerase

Ekaterina Semenova, Yulia Yuzenkova, Jean Peduzzi, Sylvie Rebuffat, Konstantin Severinov

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Peptide microcin J25 (MccJ25) inhibits bacterial RNA polymerase. We show that thermolysin-cleaved MccJ25 and MccJ25 lacking amino acids 13 to 17 also inhibit transcription. Our data and structural analysis of intact and thermolysin-digested MccJ25 suggest that distinct regions of MccJ25 are involved in transcription inhibition and cell entry.

Original languageEnglish
Pages (from-to)3859-3863
Number of pages5
JournalJournal of Bacteriology
Volume187
Issue number11
DOIs
Publication statusPublished - Jun 2005
Externally publishedYes

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