Structural features of the tmRNA-ribosome interaction

Elizaveta Y. Bugaeva, Serhiy Surkov, Andrey V. Golovin, Lars Göran Öfverstedt, Ulf Skoglund, Leif A. Isaksson, Alexey A. Bogdanov, Olga V. Shpanchenko, Olga A. Dontsova

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Trans-translation is a process which switches the synthesis of a polypeptide chain encoded by a nonstop messenger RNA to the mRNA-like domain of a transfer-messenger RNA (tmRNA). It is used in bacterial cells for rescuing the ribosomes arrested during translation of damaged mRNA and directing this mRNA and the product polypeptide for degradation. The molecular basis of this process is not well understood. Earlier, we developed an approach that allowed isolation of tmRNA-ribosomal complexes arrested at a desired step of tmRNA passage through the ribosome. We have here exploited it to examine the tmRNA structure using chemical probing and cryo-electron microscopy tomography. Computer modeling has been used to develop a model for spatial organization of the tmRNA inside the ribosome at different stages of trans-translation. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish
Pages (from-to)2312-2320
Number of pages9
JournalRNA
Volume15
Issue number12
DOIs
Publication statusPublished - Dec 2009
Externally publishedYes

Keywords

  • Chemical probing
  • Cryo-EM tomography
  • E.coli tmRNA
  • Structure modeling
  • Trans-translation

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