Streptolydigin-resistant mutants in an evolutionarily conserved region of the β' subunit of Escherichia coli RNA polymerase

K. Severinov, D. Markov, E. Severinova, V. Nikiforov, R. Landick, S. A. Darst, A. Goldfarb

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

Mutations conferring streptolydigin resistance onto Escherichia coli RNA polymerase have been found exclusively in the β subunit (Heisler, L. M., Suzuki, H., Landick, R., and Gross, C. A. (1993) J. Biol. Chem. 268, 25369- 25375). We report here the isolation of a streptolydigin-resistant mutation in the E. coli rpoC gene, encoding the β' subunit. The mutation is the Phe793 → Ser substitution, which occurred in an evolutionarily conserved segment of the β' subunit. The homologous segment in the eukaryotic RNA polymerase II largest subunit harbors mutations conferring α-amanitin resistance. Both streptolydigin and α-amanitin are inhibitors of transcription elongation. Thus, the two antibiotics may inhibit transcription in their respective systems by a similar mechanism, despite their very different chemical nature.

Original languageEnglish
Pages (from-to)23926-23929
Number of pages4
JournalJournal of Biological Chemistry
Volume270
Issue number41
DOIs
Publication statusPublished - 1995
Externally publishedYes

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