Stable stealth function for hollow polyelectrolyte microcapsules through a poly(ethylene glycol) grafted polyelectrolyte adlayer

Uta Wattendorf, Oliver Kreft, Marcus Textor, Gleb B. Sukhorukov, Hans P. Merkle

Research output: Contribution to journalArticlepeer-review

100 Citations (Scopus)

Abstract

Prospective biomedical applications of hollow polyelectrolyte microcapsules, for example, as drug delivery systems, require surface modifications that help to escape clearance by the mononuclear phagocytic system (MPS). Layer-by-layer assembled microcapsules that were alternatingly composed of polystyrene sulfonate (PSS) and polyallylamine hydrochloride (PAH) were coated with adlayers of poly(ethylene glycol) (PEG)-grafted p0ly-L-lysine (PLL-g-PEG) and p0ly-L-glutamic acid (PGA-g-PEG). Their effects on MPS recognition were studied in primary cell cultures of human monocyte derived dendritic cells and macrophages. PGA-g-PEG coatings had no significant effect on cellular recognition, which may be explained by insufficient PEG density of the adlayer. Contrary, PLL-g-PEG effectively blocked phagocytosis of coated microcapsules. In addition, PLL-g-PEG coatings showed efficient adlayer stability for at least 3 weeks, and PAH/PSS microcapsules did not impair phagocyte viability. Our results demonstrate that layer-by-layer assembled polyelectrolyte microcapsules coated with a PEG-grafted polyelectrolyte, PLL-g-PEG, represent a promising platform for a drug delivery system that escapes fast clearance by the MPS.

Original languageEnglish
Pages (from-to)100-108
Number of pages9
JournalBiomacromolecules
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 2008
Externally publishedYes

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