Stable DNA opening within open promoter complexes is mediated by the RNA polymerase β′-jaw domain

Siva R. Wigneshweraraj, Patricia C. Burrows, Konstantin Severinov, Martin Buck

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

DNA opening for transcription-competent open promoter complex (OC) formation by the bacterial RNA polymerase (RNAP) relies upon a complex network of interactions between the structurally conserved and flexible modules of the catalytic β and β′-subunits, RNAP-associated σ-subunit, and the DNA. Here, we show that one such module, the β′-jaw, functions to stabilize the OC. In OCs formed by the major σ70- RNAP, the stabilizing role of the β′-jaw is not restricted to any particular melted DNA segment. In contrast, in OCs formed by the major variant σ54-RNAP, the β′-jaw and a conserved σ54 regulatory domain co-operate to stabilize the melted DNA segment immediately upstream of the transcription start site. Clearly, regulated communication between the mobile modules of the RNAP and the functional domain(s) of the σ subunit is required for stable DNA opening.

Original languageEnglish
Pages (from-to)36176-36184
Number of pages9
JournalJournal of Biological Chemistry
Volume280
Issue number43
DOIs
Publication statusPublished - 28 Oct 2005
Externally publishedYes

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