Specificity of SNP detection with molecular beacons is improved by stem and loop separation with spacers

Valentina M. Farzan, Mikhail L. Markelov, Alexander Yu Skoblov, German A. Shipulin, Timofei S. Zatsepin

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)

    Abstract

    Molecular beacons (MBs) are valuable tools in molecular biology, clinical diagnostics and analytical chemistry. Here we describe a novel approach for the design of MBs with nucleotide or non-nucleotide linkers between the stem and loop regions. Such modified MBs have significantly improved specificity and performance for single nucleotide polymorphism (SNP) detection. These advantages are especially distinct, when compared to the classic MBs, in the case of possible interactions between the stem and loop regions. We demonstrated the applicability of such modified MBs for the discrimination of common Factor V, NOS3 and ADRB2 SNPs in model plasmids and in clinical samples. The developed approach could be applicable not only to fluorescently labeled MBs, but also to other biosensors based on nucleic acids with stem-loop structures.

    Original languageEnglish
    Pages (from-to)945-950
    Number of pages6
    JournalAnalyst
    Volume142
    Issue number6
    DOIs
    Publication statusPublished - 21 Mar 2017

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