Simple recommendations for improving efficiency in generating genome-edited mice

O. A. Averina, M. Y. Vysokikh, O. A. Permyakov, P. V. Sergiev

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The generation of transgenic model organisms (primarily mice) is an integral part of modern fundamental and applied research. Simple techniques based on the biology of these laboratory rodents can often increase efficiency when generating genome-edited mouse strains. In this study, we share our three years of experience in the optimization of mouse genome editing based on microinjection of CRISPR/Cas9 components into ca. 10,000 zygotes. We tested a number of techniques meant to improve efficiency in generating knockout mice, such as optimization of the superovulation method and choosing the optimal mouse strains to be used as zygote donors and foster mothers. The presented results might be useful to laboratories aiming to quickly and efficiently create new mouse strains with tailored genome editing.

Original languageEnglish
Pages (from-to)42-50
Number of pages9
JournalActa Naturae
Volume12
Issue number1
DOIs
Publication statusPublished - 2020
Externally publishedYes

Keywords

  • Genome editing
  • Mouse foster mothers
  • Mouse zygote donors
  • Superovulation
  • Transgenic mice

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