Simple atmospheric hydrogen/deuterium exchange method for enumeration of labile hydrogens by electrospray ionization mass spectrometry

Yury Kostyukevich, Alexey Kononikhin, Igor Popov, Eugene Nikolaev

    Research output: Contribution to journalArticlepeer-review

    74 Citations (Scopus)

    Abstract

    A simple method for hydrogen/deuterium exchange in a standard electrospray (ESI) ionization source is presented. In this method, a D2O droplet is placed between the ESI needle and the entrance of the mass spectrometer and thus saturation of the atmosphere with deuterated vapor in the ESI region is achieved. It was shown that full exchange of up to 23 labile acidic hydrogens with a minimal back exchange with the surrounding atmospheric water can be performed by this method.

    Original languageEnglish
    Pages (from-to)5330-5334
    Number of pages5
    JournalAnalytical Chemistry
    Volume85
    Issue number11
    DOIs
    Publication statusPublished - 4 Jun 2013

    Fingerprint

    Dive into the research topics of 'Simple atmospheric hydrogen/deuterium exchange method for enumeration of labile hydrogens by electrospray ionization mass spectrometry'. Together they form a unique fingerprint.

    Cite this