Sensors for Caspase Activities

O. A. Zlobovskaya, M. V. Shirmanova, T. F. Kovaleva, K. S. Sarkisyan, E. V. Zagaynova, K. A. Lukyanov

Research output: Contribution to journalReview articlepeer-review

2 Citations (Scopus)

Abstract

Cysteine proteases, caspases, play an important role in the process of programmed cell death. In apoptosis, the cell signaling pathways overlap at executioner caspases regardless of the initial stimulus. Caspase-3 is the key one. This review considers the sensors for activity of caspase-3 and some other caspases. Over the past decade, many such sensors utilizing a variety of detection principles have been created; fluorescence and luminescence are the most common detection methods. The intracellular sensors are of particular interest; they allow visualization of the activation of caspases in living systems. Herein, we briefly describe sensors of various designs, the advantages and disadvantages of which must be taken into account when choosing a sensor for a particular experimental system.

Original languageEnglish
Pages (from-to)645-652
Number of pages8
JournalRussian Journal of Bioorganic Chemistry
Volume44
Issue number6
DOIs
Publication statusPublished - 1 Nov 2018
Externally publishedYes

Keywords

  • apoptosis
  • caspase
  • fluorescent protein
  • Förster resonance energy transfer
  • luciferase
  • sensor

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