## Abstract

Self-diffusion of water-soluble fullerene derivative (WSFD) C _{60} [S(CH _{2} ) _{3} SO _{3} Na] _{5} H in mouse red blood cells (RBC) was characterized by ^{1} H pulsed field gradient NMR technique. It was found that a fraction of fullerene molecules (~13% of the fullerene derivative added in aqueous RBC suspension) shows a self-diffusion coefficient of (5.5 ± 0.8)·10 ^{−12} m ^{2} /s, which is matching the coefficient of the lateral diffusion of lipids in the erythrocyte membrane (D _{L} = (5.4 ± 0.8)·10 ^{−12} m ^{2} /s). This experimental finding evidences the absorption of the fullerene derivative by RBC. Fullerene derivative molecules are also absorbed by RBC ghosts and phosphatidylcholine liposomes as manifested in self-diffusion coefficients of (7.9 ± 1.2)·10 ^{−12} m ^{2} /s and (7.7 ± 1.2)·10 ^{−12} m ^{2} /s, which are also close to the lateral diffusion coefficients of (6.5 ± 1.0)·10 ^{−12} m ^{2} /s and (8.5 ± 1.3)·10 ^{−12} m ^{2} /s, respectively. The obtained results suggest that fullerene derivative molecules are, probably, fixed on the RBC surface. The average residence time of the fullerene derivative molecule on RBC was estimated as 440 ± 70 ms. Thus, the pulsed field gradient NMR was shown to be a versatile technique for investigation of the interactions of the fullerene derivatives with blood cells providing essential information, which can be projected on their behavior in-vivo after intravenous administration while screening as potential drug candidates.

Original language | English |
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Pages (from-to) | 1537-1543 |

Number of pages | 7 |

Journal | Biochimica et Biophysica Acta - Biomembranes |

Volume | 1860 |

Issue number | 8 |

DOIs | |

Publication status | Published - Aug 2018 |

## Keywords

- Erythrocyte membrane
- Phosphatidylcholine liposomes
- Pulsed field gradient NMR
- RBC ghost
- Self-diffusion coefficient
- Water-soluble fullerene derivative